A Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction
Author: Jianghui Hou
Publsiher: Academic Press
Total Pages: 420
Release: 2020-04-24
Genre: Science
ISBN: 9780128226438

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A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels

A Laboratory Guide to Human Physiology

A Laboratory Guide to Human Physiology
Author: Stuart Ira Fox
Publsiher: Unknown
Total Pages: 323
Release: 1984
Genre: Human physiology
ISBN: 0697047245

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Claudins

Claudins
Author: Kursad Turksen
Publsiher: Humana Press
Total Pages: 447
Release: 2011-07-02
Genre: Science
ISBN: 1617791849

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Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles. In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.

A Laboratory Guide to the Mammalian Embryo

A Laboratory Guide to the Mammalian Embryo
Author: David K. Gardner,Michelle Lane,Andrew J. Watson
Publsiher: Oxford University Press
Total Pages: 394
Release: 2004-01-08
Genre: Science
ISBN: 0195142268

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Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.

The Paracellular Channel

The Paracellular Channel
Author: Jianghui Hou
Publsiher: Academic Press
Total Pages: 248
Release: 2018-06-08
Genre: Science
ISBN: 9780128146361

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The Paracellular Channel: Biology, Physiology and Disease serves as the first volume to offer a cohesive and unifying picture of the critical functions of paracellular channels (tight junctions) in different tissues. This new class of ion channel utilizes a completely different mechanism to create ion passage pathways across the cell junction. This volume outlines common principles that govern the organization and regulation of these diverse cellular structures, describes the methodology of study, and highlights the pathophysiologic consequence of abnormal structure and functions of the paracellular channels in human diseases. Coverage includes biochemical, biophysical, structural, physiologic analyses of the paracellular channel, and new technologies for recording and characterization. Offers integrated coverage of all key aspects of the paracellular channel, an understudied field that may hold key insights into some of the most mysterious aspects of physiology Targets different levels of expertise, spanning from graduate students, interns and clinical fellows, to seasoned researchers that study functions, regulation and dysfunctions of different tissue barriers Provides a cohesive and unifying picture that describes the critical functions of paracellular channels (tight junctions) in different tissues

Human Stem Cell Manual

Human Stem Cell Manual
Author: Suzanne Peterson,Jeanne F. Loring
Publsiher: Academic Press
Total Pages: 656
Release: 2012-10-22
Genre: Science
ISBN: 9780123854742

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This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications. Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America. Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs.

Oral Histology Laboratory Guide

Oral Histology Laboratory Guide
Author: Anonim
Publsiher: Unknown
Total Pages: 73
Release: 1978
Genre: Histology
ISBN: UOM:39015060286641

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The Impact of Food Bioactives on Health

The Impact of Food Bioactives on Health
Author: Kitty Verhoeckx,Paul Cotter,Iván López-Expósito,Charlotte Kleiveland,Tor Lea,Alan Mackie,Teresa Requena,Dominika Swiatecka,Harry Wichers
Publsiher: Springer
Total Pages: 338
Release: 2015-04-29
Genre: Technology & Engineering
ISBN: 9783319161044

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“Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.

Human Neuroanatomy

Human Neuroanatomy
Author: J. Edward Bruni,Donald G. Montemurro
Publsiher: Oxford University Press, USA
Total Pages: 342
Release: 2009
Genre: Medical
ISBN: 9780195371420

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The Human Brain in Dissection will significantly update the previous edition published in 1988. The last 20 years have sen a significant shift in the way that neuroanatomy is taught in both undergraduate and graduate neuroscience courses, as well as doctorate courses: not only has the time allocated for these courses been reduced, but the methodologies for teaching have become more focused and specific due to these time constraints. The Human Brain in Dissection, Third Edition will provide detailed features of the human brain with the above limitations in mind. 50 new plates will be added to the existing 123 in order to permit the student to see all salient structures and to visualize microscopic structures of the brain stem and spinal cord. Each chapter will cover a specific are of the human brain in such a way that each chapter can be taught in one two-hour neuroanatomy course. New to this edition is the inclusion of a section in each chapter on clinically relevant examples. Each chapter will also include a specific laboratory exercise. And finally, the author has included a question and answer section that is relevant to the USMLE, as as recommended readings, neither of which were included in the previous editions. This new edition of The Human Brain in Dissection will allow the student to: understand basic principles of cellular neuroscience; learn gross and microscopic anatomy of the central nervous system (Brain, brainstem, and spinal cord); relate the anatomy of central neural pathways to specific functional systems; be able to localize and name a CNS legion when presented with neurological symptoms, and appreciate higher cortical functions and how they relate to the practice of neurology. neuroscience

NIH Guide for Grants and Contracts

NIH Guide for Grants and Contracts
Author: National Institutes of Health (U.S.)
Publsiher: Unknown
Total Pages: 135
Release: 2022
Genre: Research grants
ISBN: STANFORD:36105219414625

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General Zoology Laboratory Guide

General Zoology Laboratory Guide
Author: Charles F. Lytle,John R. Meyer
Publsiher: McGraw-Hill Science, Engineering & Mathematics
Total Pages: 372
Release: 2004-02-28
Genre: Science
ISBN: 007234900X

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General Zoology Laboratory Manual is ideal for the laboratory that emphasizes the dissection and microscopic study of live and preserved specimens. Recognized for its accuracy and readability, this manual is comprehensive in its representation of the major groups of animal phyla.This new edition is suitable for a wide range of course needs and structures.

Molecular Biology of the Cell

Molecular Biology of the Cell
Author: Bruce Alberts
Publsiher: Unknown
Total Pages: 135
Release: 2004
Genre: Electronic Book
ISBN: 0815332181

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A Comprehensive Guide to Toxicology in Nonclinical Drug Development

A Comprehensive Guide to Toxicology in Nonclinical Drug Development
Author: Ali S. Faqi
Publsiher: Academic Press
Total Pages: 986
Release: 2016-11-03
Genre: Medical
ISBN: 9780128036211

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A Comprehensive Guide to Toxicology in Nonclinical Drug Development, Second Edition, is a valuable reference designed to provide a complete understanding of all aspects of nonclinical toxicology in the development of small molecules and biologics. This updated edition has been reorganized and expanded to include important topics such as stem cells in nonclinical toxicology, inhalation and dermal toxicology, pitfalls in drug development, biomarkers in toxicology, and more. Thoroughly updated to reflect the latest scientific advances and with increased coverage of international regulatory guidelines, this second edition is an essential and practical resource for all toxicologists involved in nonclinical testing in industry, academic, and regulatory settings. Provides unique content that is not always covered together in one comprehensive resource, including chapters on stem cells, abuse liability, biomarkers, inhalation toxicology, biostatistics, and more Updated with the latest international guidelines for nonclinical toxicology in both small and large molecules Incorporates practical examples in order to illustrate day-to-day activities and the expectations associated with working in nonclinical toxicology

Strengthening Forensic Science in the United States

Strengthening Forensic Science in the United States
Author: National Research Council,Division on Engineering and Physical Sciences,Committee on Applied and Theoretical Statistics,Policy and Global Affairs,Committee on Science, Technology, and Law,Committee on Identifying the Needs of the Forensic Sciences Community
Publsiher: National Academies Press
Total Pages: 348
Release: 2009-07-29
Genre: Law
ISBN: 9780309142397

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Scores of talented and dedicated people serve the forensic science community, performing vitally important work. However, they are often constrained by lack of adequate resources, sound policies, and national support. It is clear that change and advancements, both systematic and scientific, are needed in a number of forensic science disciplines to ensure the reliability of work, establish enforceable standards, and promote best practices with consistent application. Strengthening Forensic Science in the United States: A Path Forward provides a detailed plan for addressing these needs and suggests the creation of a new government entity, the National Institute of Forensic Science, to establish and enforce standards within the forensic science community. The benefits of improving and regulating the forensic science disciplines are clear: assisting law enforcement officials, enhancing homeland security, and reducing the risk of wrongful conviction and exoneration. Strengthening Forensic Science in the United States gives a full account of what is needed to advance the forensic science disciplines, including upgrading of systems and organizational structures, better training, widespread adoption of uniform and enforceable best practices, and mandatory certification and accreditation programs. While this book provides an essential call-to-action for congress and policy makers, it also serves as a vital tool for law enforcement agencies, criminal prosecutors and attorneys, and forensic science educators.

The Laboratory Guide

The Laboratory Guide
Author: Sir Arthur Herbert CHURCH
Publsiher: Unknown
Total Pages: 292
Release: 1894
Genre: Chemistry
ISBN: WISC:89043723543

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