Lipoprotein Protocols

Lipoprotein Protocols
Author: Jose M. Ordovas
Publsiher: Humana Press
Total Pages: 284
Release: 2010-11-10
Genre: Science
ISBN: 1617370312

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The development of new methodologies has played a key role in the advancement of all areas of research. Specifically, the initial advances in our understanding of lipoprotein structure and metabolism were made possible by the development of ultracentrifugation and electrophoretic techniques. More recently, the advent of molecular biological techniques opened possibilities that were unthinkable just a few decades ago. The use of the analytical ult- centrifuge to study plasma lipoproteins began in the 1940s with the work of Mutzenbecher, McFarlene, Pedersen, Gofman, Lindgren, and Elliot. Another crucial step, during the 1950s, was the development of this tool as a prepa- tive technique by Havel, Eder, and Bragdon, among others. This technolo- cal progress allowed investigators to "dig" deeper into the structure of these complex macromolecules made of lipids and proteins, and permitted inves- gators to continue unraveling the physical and chemical characteristics of the proteins associated with lipoprotein particles (apolipoproteins) and the enzymes involved in their processing. This information led to both a better understanding of the biological functions of the lipoprotein fractions and their constituents, and creation of a more comprehensive overall scheme for plasma lipoprotein metabolism. Several gaps in this puzzle were filled through the work of Goldstein and Brown, who elucidated the structure and role of the low-density lipoprotein - ceptor. This was the first identified among a profusion of receptors that are key for the cellular catabolism of these particles.

Handbook of Lipoprotein Testing

Handbook of Lipoprotein Testing
Author: Nader Rifai,G. Russell Warnick,Marek H. Dominiczak
Publsiher: Amer. Assoc. for Clinical Chemistry
Total Pages: 819
Release: 2000
Genre: Medical
ISBN: 1890883352

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Lipoprotein Protocols Methods in Molecular Biology

Lipoprotein Protocols  Methods in Molecular Biology
Author: Jose M. Ordovas
Publsiher: Unknown
Total Pages: 135
Release: 1998
Genre: Electronic Book
ISBN: OCLC:748540464

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In Lipoprotein Protocols, Jose Ordovas compiles a cutting-edge collection of molecular protein techniques for studying advanced aspects of lipoprotein structure and metabolism. These step-by-step methods allow both the beginning and the advanced researcher to carry out not only fundamental, but also the most advanced genetic screening techniques, including RT-PCR and the preparation of relevant models of transgenic and knockout mice. In addition, novel techniques are presented to characterize plasma lipoproteins, their subfractions, and their modifications, and to determine the activity of relevant enzymes and carrier proteins. Each method is described in easily reproducible detail by hands-on experts who have perfected it. Lipoprotein Protocols offers many powerful tools for unraveling the complexity of lipoprotein metabolism and determining the individual risk of coronary heart disease. For all those basic and clinical investigators seeking to advance the frontier of lipoprotein research and develop novel genetic therapies for the treatment of cardiovascular disease, this eminently useful book constitutes the new standard reference and guide.

Flavoprotein Protocols

Flavoprotein Protocols
Author: Steven K. Chapman,Graeme A. Reid
Publsiher: Springer Science & Business Media
Total Pages: 256
Release: 2008-02-03
Genre: Science
ISBN: 9781592592661

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As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.

2 D Proteome Analysis Protocols

2 D Proteome Analysis Protocols
Author: Andrew J. Link
Publsiher: Springer Science & Business Media
Total Pages: 601
Release: 2008-02-02
Genre: Science
ISBN: 9781592595846

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With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

NMDA Receptor Protocols

NMDA Receptor Protocols
Author: Min Li
Publsiher: Springer Science & Business Media
Total Pages: 196
Release: 2008-02-03
Genre: Medical
ISBN: 9781592596836

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Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane signaling receptors. NMDA Receptor Protocols offers today's scientists powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.

T Cell Protocols

T Cell Protocols
Author: Kelly P. Kearse
Publsiher: Springer Science & Business Media
Total Pages: 365
Release: 2000
Genre: Medical
ISBN: 9781592596829

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In T Cell Protocols: Development and Activation, Kelly P. Kearse and a panel of laboratory experts describe the step-by-step execution of a wide variety of basic and specialized techniques for the study of T cell developmental biology and of their subsequent activation. Readily reproducible, the techniques are explained concisely, but in such detail that they are suitable for both the novice and experienced investigator. Coverage includes treatment of both fundamental and novel experimental protocols for the analysis of the role T cells play in immune function and in the prevention and cure of human diseases. Practical and timely, T Cell Protocols: Development and Activation offers basic scientists and clinical investigators a valuable compendium of key protocols that will improve skills with existing methods, as well as afford rapid mastery of powerful new techniques.

Adhesion Protein Protocols

Adhesion Protein Protocols
Author: Elisabetta Dejana,Monica Corada
Publsiher: Springer Science & Business Media
Total Pages: 229
Release: 1999
Genre: Science
ISBN: 9781592592586

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In Adhesion Protein Protocols, an international corps of expert investigators describe their optimized techniques for both the identification of new cell adhesion proteins and the characterization of novel adhesive structures. Their cutting edge methods will enable both novice and experienced researchers readily to identify and clone new adhesion proteins, to successfully produce inhibitors of adhesive activity, to develop biological models for the assay of cell-to-matrix and cell-to-cell adhesion in vitro, and to measure the signaling activities of adhesion proteins. The protocols described here constitute a guide to some of the most important assays in cell biology, promising significant results not only to those interested in adhesion processes, but also to scientists from many different disciplines. Adhesion Protein Protocols is the first collection of methodologies devoted entirely to the study of adhesion molecules. By illuminating these molecules and the possibilities for manipulating them, the new experimental strategies collected here offer powerful approaches to the regulation of immunity, inflammation, tissue remodeling, and embryonic development. The book will become the standard source for those wishing to discover new adhesion molecules and to characterize their biological activity.